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. 2021 Mar 21;49(7):3841–3855. doi: 10.1093/nar/gkab184

Figure 7.

Figure 7.

pBAF still forms a ternary complex with both lamin A/C and emerin. (A) ITC analysis of the interactions between pBAF and the lamin LamIgF or the emerin LEM fragments. pBAF (or BAF for comparison) being in the cell, the fragment LamIgF or LEM was injected and the heat signal was measured as a function of the injection number. Experiments were performed in duplicate, as detailed in Supplementary Table S2. (B) X-ray structure of pBAF bound to the LEM domain of emerin. The 3D structure of the complex is displayed as a cartoon, pBAF being in pink and the LEM domain in green. It is superimposed with that of BAF bound to the LEM domain (in grey; PDB 6GHD; (29)). The side chains of residues Thr3, Ser4 and Lys72 are displayed in sticks in both complexes. The specified RMSD value was calculated between all atoms of the pBAF-LEM and BAF-LEM complexes. The statistics of the pBAF-LEM structure are presented in Supplementary Table S1. (C) Zoom view on the electron density map defining the position of the side chains of pThr3, pSer4 and Lys72 in pBAF.