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Covalent attachment of XRN1 onto UV/O3 activated PMMA. (A) Schematic representation of the process of covalent attachment of XRN1 onto PMMA surface by EDC/NHS coupling reaction. 5 μm × 5 μm AFM image of PMMA surface after UV/O3 activation, and incubation with 40 nM XRN1 enzyme (B) without (C) with EDC/NHS coupling reagents. (D) Height distribution of surface features present on (C). The average height of a surface structure is 8.4 ± 0.5 nm.
