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. 2021 Jan 28;49(7):e41. doi: 10.1093/nar/gkab001

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Digestion of methylated RNA sequences. Chemical structures of (A) m6A and (B) m5C. Digestion of methylated RNA sequences by (C) solution phase and (D) immobilized XRN1. (1) Ladder (L). negative control for (2) unmethylated (c–) (4) m6A-methylated (m6A–) and (6) m5C-methylated (m5C–) RNA. Digestion results for (3) unmethylated (c+) (5) m6A-methylated (m6A+) and (7) m5C-methylated (m5C+) RNA by XRN1.