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. 2021 Jan 28;49(7):e41. doi: 10.1093/nar/gkab001

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Solution phase clipping rate and processivity of XRN1. (A) Schematic representation of the reaction procedure. (B) Fluorescence intensity of RiboGreen labelled FLuc RNA with time. According to the average length of FLuc RNA fragment remaining after the reaction (Δ nt_ave), the processivity of XRN1 in solution phase is 1113 ± 132 nucleotides. (C) Clipping rate calculated using the fluorescence decay portion from 5A. According to the slope of the graph (R² = 0.99121), the average clipping rate of XRN1 in solution is 3.06 ± 0.11 nt s^–1 at 25°C.