Figure 2.

N-terminus contains critical NLS. (A) Rif1 domain map as in Figure 1A. Schematic of Rif1 constructs tested in this figure (Blue: Rif1; Red: 6xFLAG; Orange: GBD; Purple: c-myc NLS; Gray bar: N- or C-terminal truncation of residues). (B) Western blot showing Rif1 (anti-FLAG antibody) and Pgk1 (anti-Pgk1 antibody, control) protein levels of indicated strains. (C) Southern blot showing 1L telomere probe for the indicated strains. (D) Southern blot from C, rehybridized with a Y’ probe to visualize ‘bulk’ XhoI restriction fragments (see ‘Materials and Methods’ section).