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. 2021 Mar 27;49(7):3967–3980. doi: 10.1093/nar/gkab206

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — HEAT repeats of Rif1 when localized to telomere are functionally sufficient in telomere regulation. (A) Rif1 domain map as in Figure 1A. Schematic of Rif1 constructs tested in this figure (Blue: Rif1; Red: 6xFLAG; Orange: GBD; Purple: c-myc NLS; Gray bar: N- or C-terminal truncation of residues). (B) Western blot showing Rif1 (anti-FLAG antibody) and Pgk1 (anti-Pgk1 antibody, control) protein levels of indicated strains. (C) Southern blot showing 1L telomere probe for the indicated strains. (D) Southern blot showing 1L telomere probe for the indicated strains. (E) Southern blot from C, rehybridized with a Y’ probe to visualize ‘bulk’ XhoI restriction fragments (see ‘Materials and Methods’ section).