Figure 7.
Overexpression of snR67 rescues 2′-O-methylation of Gm2619 and Um2724 when Dbp3 is lacking. (A, B) RNA from wild type yeast or strains lacking Dbp3 transformed with an empty pRS416 vector (EV) or a plasmid for the overexpression of snR67 from a pGAL1 promoter (snR67OE), or a strain in which SNR67 was deleted (Δ67) was subjected to RNase H (RH)-mediated cleavage targeting 25S-Gm2619 (A) or 25S-Um2724 (B). Full length 25S rRNA and cleavage products (indicated by arrow head) were detected by northern blotting using probes hybridising downstream of the cleavage sites. Asterisks indicate non-specific cleavage products – note that the extent of non-specific RNase H-mediated cleavage is reduced upon cleavage at a specific cleavage site.