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. 2021 Mar 15;49(7):3781–3795. doi: 10.1093/nar/gkab154

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Overexpression of Sgf29-FLAG confers resistance to TSA during development. (A) Schematic illustration of wildtype and F359A/Y366A Sgf29-FLAG overexpression constructs. The expression of C-terminally FLAG-tagged protein is driven by the actin 15 promoter. (B) Overexpressing Sgf29-FLAG in Ax2 cells confers resistance to TSA during development. Upper panel, immunoblots showing expression of FLAG-tagged wildtype and F359A/Y366A Sgf29 in Ax2 cells. Whole cell lysates from pools of cells transfected with empty vector (EV), or constructs to drive expression of wildtype and F359A/Y366A Sgf29 were resolved by 12% SDS-PAGE and blotted using an anti-FLAG antibody. Actin is used as a loading control. Lower panel, cells were washed with KK2 and allowed to develop on solid agar with increasing concentration of TSA as described in Figure 1A. Images were taken at 24 h and representative images of three repeats shown. Lower panel, the percentage of late structures is shown as average ± SD from three independent experiments. Statistical significance was calculated using a two-way ANOVA with Tukey test. *P < 0.05; **P < 0.01; ****P < 0.0001.