Figure 1.

Effects of pHNSHP45-encoded ProQ/FinO family protein PcnR on bacterial growth and fitness cost. (A) Effects of pHNSHP45 encoded putative regulators on bacterial growth. These data represent the mean of three independent experiments. (B) Relative fitness of E. coli BW25113 harbouring pHNSHP45Δorf00050::kan, pHNSHP45Δorf00039::kan, pHNSHP45ΔpcnR::kan, and its complementation strain pHNSHP45ΔpcnR::kan-pHSG575-pcnR in vitro competition. The reference strain is BW25113/pHNSHP45. Complementation assays were performed by expressing pcnR from its native promoter on pHSG575-pcnR. All competitions assays were carried out with three biological replicates and the relative fitness of each strain was detected at 24, 48 and 72 h. (C) The colonial morphology of E. coli BW25113/pHNSHP45 and BW25113/pHNSHP45ΔpcnR on LB agar plate.