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. 2021 Mar 15;49(7):3981–3996. doi: 10.1093/nar/gkab149

Figure 6.

Figure 6.

Bacterial population dynamics in co-cultures with plasmid-free and plasmid-containing E. coli BW25113. Plasmid-containing strains including E. coli BW25113/pHNSHP45, BW25113/pHNSHP45ΔpcnR::kan, BW25113/pHNSHP45Δmcr-1::kan,BW25113/pHNSHP45ΔpcnRΔmcr-1::kan were mixed with a 1000-fold excess of plasmid-free E. coli BW25113 at the beginning of the invasion assay. E. coli BW25113 is indicated by black dot, BW25113/pHNSHP45 is indicated by green dot, BW25113/pHNSHP45Δmcr-1::kan is indicated by blue dot, BW25113/pHNSHP45ΔpcnR::kan is indicated by orange dot, and BW25113/pHNSHP45ΔpcnRΔmcr-1::kan is indicated by red dot. (A) Co-cultures with E. coli BW25113 and BW25113/pHNSHP45. (B) Co-cultures with E. coli BW25113 and BW25113/pHNSHP45ΔpcnR::kan. (C) Co-cultures with E. coli BW25113 and BW25113/pHNSHP45Δmcr-1::kan. (D) Co-cultures with E. coli BW25113 and BW25113/pHNSHP45ΔpcnRΔmcr-1::kan. (E) Co-cultures with E. coli BW25113, BW25113/pHNSHP45, and BW25113/pHNSHP45ΔpcnR::kan. (F) Co-cultures with E. coli BW25113, BW25113/pHNSHP45 and BW25113/pHNSHP45Δmcr-1::kan. (G) Co-cultures with E. coli BW25113, BW25113/pHNSHP45, and BW25113/pHNSHP45ΔpcnRΔmcr-1::kan. Bars represent SD of three biological replicates.