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. 2021 Mar 21;49(7):3814–3825. doi: 10.1093/nar/gkab181

Figure 3.

Figure 3.

PuREBP1 and PuREBP2 bind to RNA in vivo. (A) Orthogonal Organic Phase Separation (OOPS) assay. Cells expressing PuREBP1-TAP or TAP-PuREBP2 were irradiated with UV light in vivo and lysed in peqGOLD TriFast reagent. Partition of proteins to the organic phase or to the interphase was monitored by immunoblot. Phosphoglycerate kinase B served as a negative control. (B) RNA immunoprecipitation (RIP) followed by quantitative RT-PCR assays. Actin (ACT) and amino-acid transporter 11 (AATP11) transcripts were used as non-bound controls. Percentages of immunoprecipitated RNA relative to input RNA are expressed as the mean ± s.e.m. (n = 4).

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