Figure 5.
Label-free MST was performed to determine the affinity between DLDC4 and DLDC8 NP variants towards 9-mer RNA (top-left and bottom-left panels, respectively). By saturating DLDC8 mutant with a synthetic tail peptide, RNA binding was attenuated by 8-fold. MST curve is expressed as Fnorm (0/00) versus concentration. Data represent mean values from at least three independent measurements. Error bars represent standard deviation. In the bottom right panel, for comparison, MST curves were expressed as fraction of protein bound vs ligand (RNA) concentration (Blue: DLDC8; Green: peptide-saturated DLDC8).