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. 2021 Mar 25;49(7):3692–3708. doi: 10.1093/nar/gkab194

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of CC-885 and CC-90009 on TP53 PTC readthrough. (A, B) HDQ-P1 cells were exposed to the indicated concentrations of CC-885 (A) or CC-90009 (B) for 48 h and p53 levels were determined. Vinculin was used as loading control. (C, D) HDQ-P1 cells were exposed to the indicated concentrations of CC-885 (C) or CC-90009 (D) in combination with 20 μg/ml G418 for 48 h and p53 levels were measured. Vinculin was used as loading control. (E, F) HDQ-P1 cells were exposed to the indicated concentrations of CC-885 (E) or CC-90009 (F) without or with 20 μg/ml G418 for 48 h and TP53 level was measured in triplicate samples (±S.D.). (G) HDQ-P1 cells were transiently transfected with transfection reagents only (Mock), FLAG-tagged eRF3a or eRF3a-G575N constructs and exposed to the indicated concentrations of CC-90009 in combination with 20 μg/ml G418 for 48 h and p53, eRF3a, eRF1 and UPF1 were measured. Vinculin and GAPDH were used as loading controls. * indicates statistically significant difference to untreated samples (P < 0.01).