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. 2021 Mar 30;49(7):4104–4119. doi: 10.1093/nar/gkab196

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Methylation changes for individual sites in fib mutants. (A) MethScores are plotted as mean ± SD (n = 3 biological replicates) for all detected Nm in WT and two fib plants. (B) Correlation (Pearson's R) between MethScore changes in fib1-1 and fib2-1. (C) Correlation between MethScore changes in fib1-1 and MethScores in WT. (D) Correlation between MethScore changes and fraction changes of cumulated abundance of guide RNAs in fib1-1. Normalized TPM of all assigned guide RNAs for a methylation site were combined to calculate fraction of change between WT and fib1-1. The values in B–D are all based on means of n = 3 biological replicates.