Table 3.

The dissociation constants of MtrR DNA-binding domain point mutants and selected DNA binding sites

	K d (nM)a
MtrR mutants	T11A	T43A	T43S	R44A	G45A	Y48F	W49F	W49A	H50A
Oligoduplexes
rpoH 27mer	441 ± 37	NDBb	860 ± 180	NDB	NSc	NS	53 ± 3	NS	376 ± 35
mtrCDE 21mer	1076 ± 93	NDB	NS	NDB	(52 ± 13)d	NS	380 ± 44	NS	990 ± 100
GpY 27mer	NS	NDB	NS	NDB	NDB	NS	NS	NS	NS
emrAB 22mer	NS	NDB	NS	NDB	NDB	NS	NS	NS	NS

^aReported values are averages and associated standard error of the mean from three separate experiments.

^bNDB = No detectable binding indicates that the data could not be fit by our binding equations.

^cNS = Nonspecific binding is designated for interactions with K_d > 1.5 μM.

^dThe measured K_d is unreliable given small change in mP; it is unlikely these data represent specific binding.