Table 3.

Comparison of immune cell subset infiltration in relation to S100A8 + immune cells

Immune cell subset	Pre-invasive carcinomaa		p value	Invasive carcinomab		p value
	S100A8+ IC (−)	S100A8+ IC (+)		S100A8+ IC (−)	S100A8+ IC (+)
Total
CD4+ TIL	15.33 (2.33–34.00)	35.00 (9.67–90.17)	< 0.001	76.00 (30.50–136.50)	127.50 (52.50–195.50)	< 0.001
CD8+ TIL	9.0 (4.33–19.33)	15.00 (8.83–41.33)	< 0.001	59.00 (29.50–110.00)	166.00 (81.50–290.50)	< 0.001
FOXP3+ TIL	0.00 (0.00–0.00)	0.00 (0.00–4.00)	0.006	5.00 (2.00–11.00)	17.00 (9.00–28.00)	< 0.001
PD-L1+ IC	9/92 (9.8)	21/82 (25.6)	0.006	38/161 (23.6)	100/146 (68.5)	< 0.001
HR+ subgroup
CD4+ TIL	14.33 (2.00–29.33)	43.00 (9.33–91.33)	< 0.001	76.50 (34.25–136.75)	109.50 (46.50–188.00)	0.023
CD8+ TIL	9.00 (4.33–17.83)	14.67 (9.00–39.00)	< 0.001	56.00 (29.00–99.50)	133.00 (54.50–277.75)	< 0.001
FOXP3+ TIL	0.00 (0.00–0.00)	0.00 (0.00–4.00)	0.012	5.00 (2.00–10.00)	15.00 (7.00–20.00)	< 0.001
PD-L1+ IC	8/85 (9.4)	15/68 (22.1)	0.030	22/132 (16.7)	45/80 (56.3)	< 0.001
HR- subgroup
CD4+ TIL	53.3 (39.17–81.08)	27.67 (18.08–84.33)	0.689	71.00 (15.00–131.50)	138.50 (63.00–203.25)	0.012
CD8+ TIL	18.50 (6.08–38.92)	19.00 (6.58–65.50)	0.585	77.00 (35.00–163.00)	195.00 (108.50–306.50)	< 0.001
FOXP3+ TIL	0.50 (0.00–5.92)	1.00 (0.00–5.75)	0.904	8.00 (3.00–11.50)	19.00 (12.00–33.25)	< 0.001
PD-L1+ IC	1/7 (14.3)	6/14 (42.9)	0.337	16/29 (55.2)	55/66 (83.3)	0.004

P values are calculated by Mann–Whitney U test or Chi-square test. Data are presented as median (interquartile range) for TIL and frequency (%) for PD-L1+ IC

TIL tumor-infiltrating lymphocyte, IC immune cell, HR hormone receptor

^aIn pre-invasive carcinomas, data on immune cell subset infiltration were missing in six cases: CD4+ TIL in one case, FOXP3+ TI in two cases, CD8+ TIL in one case, PD-L1+ IC in two cases

^bIn invasive carcinomas, data on immune cell subset infiltration were missing in three cases: CD8+ TIL in one case, FOX3+ TIL in one case, and PD-L1+ IC in one case