Fig. 4.

Real-time PCR analysis of HAMP and ELISA measurements of secreted hepcidin of LPS and LTA treated BV2 cells. Quantitative Real-Time PCR analysis was performed using gene specific primers and SYBR Green method. The expression level of the gene of interest was compared to the level of the β-actin in each sample. The mRNA expressions of the treated cells were compared to the appropriate untreated controls (9 h or 24 h). The relative expression of the controls was regarded as 1. Hepcidin content of cell culture supernatants was determined with Mouse hepcidin-25 ELISA Kit. a, b mRNA levels of HAMP. c, d Secreted hepcidin concentrations of LPS and LTA treated BV2 cells. The columns represent mean values and error bars represent standard deviation (SD) of three independent determinations (n = 3). The asterisk indicates p < 0.05 compared to the untreated controls. Data was analysed by two-way ANOVA followed by Tukey’s HSD post hoc test