Fig. 9.

Changes of the NFκB signalling pathway after neutralization of IL-6 in LPS and LTA treated BV2 cells. The treated cells were collected and fractionated. Protein contents of the fractions were measured and the same amount of protein from each sample was loaded and separated onto 12% polyacrylamide gels, then were transferred by electroblotting onto nitrocellulose membranes. The membranes were probed with rabbit polyclonal antibodies produced against p50 and p65 according to the manufacturer’s protocols. The β-actin was used as housekeeping control. a Western blot analysis of p50 and p65 in the chromatin-bound nuclear fractions of LPS and LTA treated BV2 cells. b Optical density analysis of p50 and p65 proteins. Optical densities were calculated by ImageJ software and expressed as percentage of target gene/β-actin abundance. The columns represent mean values and error bars represent standard deviation (SD) of three independent experiments (n = 3). Ctrl untreated control, NCtrl control + neutralizing IL-6 antibody, NLPS neutralizing IL-6 antibody + LPS, NLTA neutralizing antibody + LTA. The asterisk marks p < 0.05 compared to Ctrl. The cross marks p < 0.05 compared to NCtrl. The double cross indicates significant difference, p < 0.05 between LPS and NLPS or LTA and NLTA. Data was analysed by two-way ANOVA followed by Tukey’s HSD post hoc test