Table 5.
Concordance of PCR Data and Results of ISH in Histologic and Cytologic Diagnoses*
| Histologic Result | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nondysplastic Tissues | CIN 1 ISH Data | CIN 2 | CIN 3 | Carcinoma | |||||||||
| Cytologic/PCR Data | HR-HPV | LR-HPV | – | HR-HPV | LR-HPV | – | HR-HPV | LR-HPV | – | HR-HPV | HR-HPV | – | Total |
| Negative LBC | |||||||||||||
| Negative | 0 | 0 | 42 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 44 |
| HR-HPV | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
| LR-HPV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ASCUS | |||||||||||||
| Negative | 4 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 11 |
| HR-HPV | 3 | 0 | 2 | 8 | 0 | 0 | 5 | 0 | 1 | 2 | 2 | 0 | 23 |
| LR-HPV | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 |
| LSIL | |||||||||||||
| Negative | 1 | 0 | 2 | 8 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 14 |
| HR-HPV | 5 | 0 | 2 | 24 | 0 | 0 | 6 | 0 | 0 | 1 | 0 | 0 | 38 |
| LR-HPV | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 |
| HSIL | |||||||||||||
| Negative | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| HR-HPV | 0 | 0 | 0 | 3 | 0 | 0 | 6 | 0 | 0 | 2 | 1 | 0 | 12 |
| LR-HPV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Carcinoma | |||||||||||||
| Negative | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
| HR-HPV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
| LR-HPV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
| Total | 15 | 1 | 52 | 48 | 2 | 3 | 21 | 0 | 3 | 6 | 4 | 1 | 156 |
| Total concordance | 8/15 (53) | 1/1 (100) | 45/52 (87) | 35/48 (73) | 1/2 (50) | 3/3 (100) | 17/21 (81) | 0/0 (0) | 2/3 (67) | 5/6 (83) | 4/4 (100) | 1/1 (100) | 122/156 (78.2) |
ASCUS, atypical squamous cells of undetermined significance; CIN, cervical intraepithelial neoplasia; HPV, human papillomavirus; HR, high-risk; HSIL, high-grade squamous intraepithelial lesion; ISH, in situ hybridization; LBC, liquid-based cytology; LR, low-risk; LSIL, low-grade squamous intraepithelial lesion; PCR, polymerase chain reaction.
Data in parentheses are number/total [percentage], except for total concordance data, which are number/total (percentage). Twenty cases positive for high- and low-risk HPV types by one method and/or unknown HPV types (by PCR) were excluded from this analysis, and only cases negative for HPV or positive for HR-HPV or LR-HPV (by PCR or ISH) were included in the analysis.