Figure 2.
Profiling of serum IgG ACAs. The serum IgG autoantibody titres against each centromere antigen were analysed by the antigen-binding bead assay with the sera of patients with SS (n=86), SSc (n=35), PBC (n=10), overlap disease (n=42), and healthy controls (HC; n=68). (A) Each symbol represents the antibody level in an individual’s serum and the dotted line indicates the cut-off value, which was determined by the median plus 5IQR of MFI in HC. (B) Bar graphs show the prevalence of autoantibodies against centromere antigens measured as MFI in each disease group. The prevalence of the discrete-speckled pattern by the ANA test and anti-CENP-B antibody by ELISA are shown in the top. *p<0.05 between each disease group and the HC group; †novel autoantigen as a form of complex, at least one component molecule is known as an autoantigen; ‡novel autoantigen identified in this assay. The data of CBX5, CENP-A, CENP-B, CENP-C, and the MIS12 complex were obtained from our previous study.17 ANA, anti-nuclear antibody; ELISA, enzyme-linked immunosorbent assay; MFI, mean fluorescence intensity.