Figure 2. Identification of G12V and G13D mKRAS specific clones by phage-ELISA at 450 nm absorbance.

After three rounds of panning, 50 individual randomly selected clones were infected with M13K07 helper phage to produce phage displayed scFv antibodies. Phage ELISA data for fourteen positive clones are shown. Clones that exhibited at least two times stronger ELISA signals than the BSA negative control (white bars) were deemed as specific clones. A total of (A) six positive clones of G13D and (B) eight positive clones of G12V were detected. Detection was performed using HRP-conjugated anti-M13 antibody and wt M13K07 helper phages were used as negative phage controls. Data were presented as mean values ± standard deviation indicated by error bars from triplicate experiments.