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. 2021 Apr 15;9:e11063. doi: 10.7717/peerj.11063

Figure 3. Western immunoblot analysis of anti-mKRAS scFvs in periplasmic fractions.

Figure 3

Fractions were resolved on 12% (w/v) SDS-PAGE, immunoblotted and reacted with anti-E-tag antibody (1:1,000). Lane M, Thermo Fisher Spectra™ Multicolor low range protein ladder; Lane 1, IPTG-uninduced anti-mKRAS G13D-5 scFv; Lane 2, IPTG- induced anti-mKRAS G13D-5 scFv; Lane 3, IPTG-uninduced anti-mKRAS G13D-18 scFv; Lane 4, IPTG-induced anti-mKRAS G13D-18 scFv; Lane 5, IPTG-uninduced anti- mKRAS G12V-34 scFv; Lane 6, IPTG-induced anti-mKRAS G12V-34 scFv; Lane 7, IPTG-uninduced anti-mKRAS G12V-50 scFv; Lane 8, IPTG-induced anti-mKRAS G12V-50 scFv. Arrows indicate the presence of a 30 kDa band of anti-mKRAS scFv.