Figure 4. Cross-reactivity of soluble anti-mKRAS scFvs with control K-ras (G13D, G12V) and wildtype K-ras antigens in monoclonal-ELISA.

Four clones (G13D-5, G13D-18, G12V-34 and G12V-50) were tested for binding specificity towards wt (light grey bars), G13D (dark grey bars) and G12V (black bars) control peptides. Wells were coated with 10 μg/mL of each antigen and bound antibodies were detected with anti E-tag antibodies (1:5,000). All values were presented as mean values ± standard deviation against background signals from plates without the addition of sera and non-coated wells, measuring at 450 nm wavelength. Significant differences were denoted as p-values < 0.05 (*) and <0.01 (**) when compared against G12V or G13D antigen controls.