Table 4. Amino acid sequences of G12V-34, G12V-50, G13D-5 and G13D-18 scFvs isolated from phage antibody libraries.
CDR, complementarity determining regions; FR, framework regions.
| scFv | FR1 | CDR1 | FR2 | CDR2 | FR3 | CDR3 | FR4 |
|---|---|---|---|---|---|---|---|
| VH sequences | |||||||
| G12V-34 | QVQLQQPGAELVRPGASVKLSCKTS | GYSFTSYW | MNWVKQRPGQGLEWIGY | INPSTGYT | EYNQKFKDKATLTADKSSSTAYMQLSSLTSEDSAVYYC | ARRDYRYFDV | WGAGTTVTVSS |
| G12V-50 | QVQLKQSGAELVKPGASVKLSCKAS | GYTFTSYW | MHWVKQRPGQGLEWIGE | IDPSDSYT | NYNQKFKDKATLTVDKSSSTAYIQLSSLTSEDSAVYYC | ARGGYFDY | WGQGTTVTVSS |
| G13D-5 | QVQLKQSGAELVKPGASVKLSCKAS | GYTFTSYW | MHWVKQRPGQGLEWIGE | IPSDSYT | NYNQKFKDKATLTVDKSSSTAYIQLSSLTSEDSAVYYC | ARGGYFDY | WGQGTTVTVSS |
| G13D-18 | GSELVRPGASVKLSCKAS | GYTFTNYW | IHWVKQRPGQGLEWIGN | VYPGTGTT | YYDEKFKSKASLTVDTSSSTAFMQLSSLTSEDSAVYYC | TRIDYESLYFGV | WGAGTTLTVSS |
| VL sequences | |||||||
| G12V-34 | DIVMTQSPALMSAALGERVTMTCSAS | SSVSY | MHWYQQKSGTSPKRWIY | DTS | KLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYC | QQWSSNPFT | FGSGTKLEIK |
| G12V-50 | DIVLTQSHKFICSSVGDRVSITCKAS | QDVSTA | VAWYQRKPGQSPKLLIY | SAS | YRCTGVPDRFTGSGSGTDYTLTISSVQAEDLALYYC | QQHYSTPFT | FGSGTKLEIK |
| G13D-5 | DIVLTQSPALMSASPGEKVTMTCSAS | SSVSY | MYWYQQKPRSSPKPWIY | LTS | NLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYC | QQWSNNPLT | FGAGTKLELK |
| G13D-18 | DIQLTQSPASLSASVGETVTITCRAS | ENIYSY | LAWYQQKQGKSPQLLVY | AAT | NLADGVPSRFSGSGSGTQYSLKINSLQSEDFGSYYC | QHFWGTPFT | FGSGTKLEIK |