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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Nat Protoc. 2020 May 13;15(6):2041–2070. doi: 10.1038/s41596-020-0320-x

Figure 7∣. Various procedures/considerations for milling.

Figure 7∣

a, GIS deposition of organo-platinum followed by the FIB induced baking away of volatile organic components. b, Milling angles and resulting lamella lengths along with a layer of GIS-deposition on the front end of the lamella. The milling angle significantly influence the length of the lamellae produced. Milling at shallower angle is particularly important for thin biological samples such as bacterial monolayer. c, A schematic representation of the evolution of milling patterns, distances between them and the FIB currents used. Two rectangular milling patterns are selected and positioned to define the area to be sputtered away. These parameters are empirical and vary significantly between different samples.