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Robustness of SAMDI-MS assay for NSP14/NSP10 ExoN activity using all cleavable substrate. Full-plate uniformity data using optimized conditions and 6 µL reaction volumes for SAMDI-MS highlight the (A) robustness measured by the Z factor and (B) assay window measured the S/B ratio. Low controls were prequenched with 100 mM EDTA (final).
