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. 2021 Apr 13;14:1631–1645. doi: 10.2147/DMSO.S298950

Figure 3.

Figure 3

The knockdown of MALAT1 inhibited pyroptosis and OS induced by HG. (A and B) MALAT1 and miR-200c mRNA were assayed by qRT-PCR. The results supported the transfection investigation. *P <0.05, **P <0.01, ****P <0.0001. (C) After transfection with MALAT1-1, 2, and 3 siRNA, qPCR assay of MALAT1 mRNA expression identified the fragment with the best transfection efficiency. *P <0.05, **P <0.01, ***P <0.001, ns, non significant. (D–F) Pyroptosis and OS were evaluated after the knockdown of MALAT1 by qPCR and Western blotting. *P <0.05, **P <0.01, ***P <0.001, ns, non significant. (G) Pyroptosis after transfection with MALAT1 was analyzed by flow cytometry. ***P <0.001. (H) GSH, SOD, and MDA levels were assayed after the transfection of MALAT1. *P <0.05, **P <0.01, ****P <0.0001, ns, non significant. (I) Levels of caspase-1, and NRF2 in cells transfected with MALAT1 were assayed by immunofluorescence. I, HG + control; II, HG + NC siRNA; III, HG + MALAT1-1 siRNA; IV, HG + MALAT1-2 siRNA; V, HG + MALAT1-3 siRNA. *P <0.05, **P <0.01, ns, non significant.