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. 2021 Apr 12;14:2475–2488. doi: 10.2147/OTT.S293180

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© 2021 Huang et al.

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MiR-516b-5p accumulation inhibits the malignant behaviors of ESCC cells. (A–E) Eca109 and KYSE150 cells were transfected with miR-NC or miR-516b-5p. (A) Cell migration ability was assessed by wound healing assay. (B and C) Cell migration and invasion abilities were analyzed by transwell migration assay and transwell invasion assay, respectively. (D) MTT assay was conducted to assess cell proliferation capacity. (E) The number of colonies was analyzed by colony formation assay. **P<0.01, ***P<0.001.