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. 2021 Apr 12;14:2475–2488. doi: 10.2147/OTT.S293180

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© 2021 Huang et al.

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Circ_0006168 interacts with miR-516b-5p in ESCC cells. (A) The interaction between circ_0006168 and miR-516b-5p was predicted by Circinteractome software. (B) The target interaction between miR-516b-5p and circ_0006168 was tested via dual-luciferase reporter assay. (C and D) RIP assay was applied to confirm the target relationship between miR-516b-5p and circ_0006168. (E) RNA pull down assay was utilized to verify the interaction between miR-516b-5p and circ_0006168. **P<0.01, ***P<0.001.