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. 2021 Apr 12;14:2463–2473. doi: 10.2147/OTT.S291403

Figure 2.

Figure 2

Silencing ACTL8 suppressed the proliferation in MDA-MB-231 and BT-549 cells. (A) ACTL8 protein expression in TNBC cells transfected with control, si-NC orsi-ACTL8 was determined by Western blot. (B) ACTL8 mRNA expression in TNBC cells transfected with control, si-NC orsi-ACTL8 was determined by qRT-PCR. (C) EdU assay was used to assess the proliferation of the transfected MDA-MB-231 and BT-549 cells. (D) Colony formation assay was executed to detect the colony numbers of the transfected MDA-MB-231 and BT-549 cells. **P < 0.01, vs Control and si-NC group.