Fig. 1.

The sTRAIL-overexpressing hTERT immortalized human adipose stem cell line (hTERT-ADSC. sTRAIL). (A) hTERT-ADSC and hTERT-ADSC.sTRAIL were generated by the lentiviral transduction of GFP and the sTRAIL gene using the CLV-Ubic vector. (B), (C): The expression of the sTRAIL transcript or protein was confirmed by RT-PCR or Western blots, respectively. (D) Phase-contrast microscopy of the hTERT-ADSC.GFP line (left) and hTERT-hADSC.GFP. sTRAIL (right) line. ADCS = hTERT-hADSC, sTRAIL = secretable trimeric forms of tumor necrosis factor (TNF)-related apoptosis-inducing ligand, ADSC = adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSC, RT-PCR = reverse transcription-polymerase chain reaction.