Table 1.
PCR primer sequences.
| Gene | Sequence | Size (bp) |
|---|---|---|
| CE | Sense: 5′-TGCTGGGCTATCCACTCTCT-3′ | 237 |
| Antisense: 5′-CTCCAGCATCTCTGTGGTGA-3′ | ||
| SCF | Sense: 5′-ACTTGGATTCTCACTTGCATTT-3′ | 505 |
| Antisense: 5′- CTTTCTCAGGACTTAATGTTGAAG-3′ | ||
| c-kit | Sense: 5′- GCCCACAATAGATTGGTATTT-3′ | 332 |
| Antisense: 5′-AGCATCTTTACAGCGACAGTC-3′ | ||
| SDF-1 | Sense: 5′-ATGAACGCCAAGGTCGTGGTC-3′ | 200 |
| Antisense: 5′-GGCTGTTGTGCTTACTTGTTT-3′ | ||
| CXCR4 | Sense: 5′-CTCTCCAAAGGAAAGCGAGGTGGACAT-3′ | 733 |
| Antisense: 5′-AGACTGTACACTGTAGGTGCTGAAATCA-3′ | ||
| VEGF | Sense: 5′-AAGCCATCCTGTGTGCCCCTGATG-3′ | 541 |
| Antisense: 5′-GCTCCTTCCTCCTGCCCGGCTCAC-3′ | ||
| VEGFR1 | Sense: 5′-GCAAGGTGTGACTTTTGTTC-3′ | 512 |
| Antisense: 5′-AGGATTTCTTCCCCTGTGTA-3′ | ||
| VEGFR2 | Sense: 5′-ACGCTGACATGTACGGTCTAT-3′ | 438 |
| Antisense: 5′-GCCAAGCTTGTACCATGTGCG-3′ | ||
| VEGFR3 | Sense: 5′-AGCCATTCATCAACAAGCCT-3′ | 298 |
| Antisense: 5′-GGCAACAGCTGGATGTCATA-3′ | ||
| β-actin | Sense: 5′-GCA CCA CAC CTT CTA CAA TG -3 Antisense: 5′-TGC TTG CTG ATC CAC ATC TG -3 |
619 |
Carboxylesterase (CE); stem cell factor (SCF); stromal cell-derived factor 1 (SDF-1); C-X-C Motif Chemokine Receptor 4 (CECR4); Vascular endothelial growth factor (VEGF); Vascular endothelial growh factor receptor (VEGFR).