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. 2021 Apr 19;7:25. doi: 10.1038/s41421-021-00265-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Distribution of different types of sRNAs extracted from HEK293T cells that we process with the full CPA-seq process or with various combinations of the Cap-Clip, T4 PNK, and AlkB mix enzymes (n = 2). b The number of sRNA species revealed with different treatments (n = 2). c Distribution of different types of sRNAs responsive to T4 PNK treatment (unique reads that were highly detected in CPA group, but lowly detected in CA group with the fold change > 30, n = 2). d Reads of sRNA responsive to T4 PNK treatment mapping to 5S, 18S, and 28S ribosomal RNAs have been combined to show detection of rsRNAs containing diverse termini (rsRNAs with RPM > 300 are shown in the structural map). e Reads of sRNA responsive to T4 PNK treatment mapping to cytosolic tsRNAs have been combined to show detection of tsRNAs containing diverse termini. f Reads of tsRNAs responsive to T4 PNK treatment. g Northern blotting of GluCTC 5′tsRNAs that are responsive to T4 PNK treatment.