Figure 3.

In vitro transfection using ascending doses of His-lPEI or bPEI polyplexes incorporating either p(3NF₁-Luc-3NF₂)m or p(Scr₁-Luc-Scr₂)m

(A–H) Experiments were carried out in SK-Mel-28 (A, B, E, and F) and C2C12 (C, D, G, and H) cells using His-lPEI (A–D) or bPEI (E–H) polyplexes. Results are mean values of three wells ± SD. ∗p ≤ 0.05 (multiple t test Holm-Sidak method) between p(3NF₁-Luc-3NF₂)m and p(Scr₁-Luc-Scr₂)m. Cell survival is given as a percentage compared to untreated cells. See Figure S5 for complementary results obtained with p(3NF₁-Luc-Scr₂)m and p(Scr₁-Luc-3NF₂)m.