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The Experiments Designed by CCD-RSM
| Factors | Symbol | Coded Levels | ||||
|---|---|---|---|---|---|---|
| −2 | −1 | 0 | 1 | 2 | ||
| BSA concentration/mg/mL | X1 | 7.5 | 15.0 | 22.5 | 30.0 | 37.5 |
| pH value | X2 | 6.5 | 7.5 | 8.5 | 9.5 | 10.5 |
| Glutaradehyde concentration/% | X3 | 25 | 75 | 130 | 175 | 225 |
| Speed of ethanol addition/mL/min | X4 | 0.5 | 1.0 | 1.5 | 2.0 | 2.5 |
Notes: Four main factors (BSA concentration, pH value, speed of ethanol addition and glutaraldehyde volume) were investigated; five levels were designed for each factor.
