Table 1.

Primers for detection of Entamoeba species.

Primer	Genus/species	Sequence (5′-3′)	Annealing temperature	Reference
E-1	Entamoeba	TAAGATGCAGAGCGAAA	58 °C	(Khairnar and Parija, 2007)
E-2	Entamoeba	GTACAAAGGGCAGGGACGTA	58 °C	(Khairnar and Parija, 2007)
EH-1	E. histolytica	AAGCATTGTTTCTAGATCTGAG	50 °C	(Khairnar and Parija, 2007)
EH-2	E. histolytica	AAGAGGTCTAACCGAAATTAG	50 °C	(Khairnar and Parija, 2007)
ED-1	E. dispar	TCTAATTTCGATTAGAACTCT	52 °C	(Khairnar and Parija, 2007)
ED-2	E. dispar	TCCCTACCTATTAGACATAGC	52 °C	(Khairnar and Parija, 2007)
Mos-1	E. moshkovskii	GAAACCAAGAGTTTCACAAC	48 °C	(Khairnar and Parija, 2007)
Mos-2	E. moshkovskii	CAATATAAGGCTTGGATGAT	48 °C	(Khairnar and Parija, 2007)
TN21	Entamoeba	AAGATTAAGCCATGCATGTSKA	58 °C	(Matey et al., 2016)
TN14	Entamoeba	GATACCTTGTTACGACTTCTY	58 °C	(Matey et al., 2016)
MA67	E. hartmanni	TTGGATGTAGAGATACATTC	54 °C	(Matey et al., 2016)
T28	E. hartmanni	CACTATTGGAGCTGGAATTAC	54 °C	(Matey et al., 2016)
MA113	E. coli	GCCAAGAGAATTGTAGAAATCG	54 °C	(Matey et al., 2016)
T28	E. coli	CACTATTGGAGCTGGAATTAC	54 °C	(Matey et al., 2016)