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Morphological changes during hPSC-OP9 coculture
(A) Morphology of overgrown OP9 stroma at 8 days post passage, ready to be seeded with hPSCs.
(B) Early hPSC (MIFF3) colony growing on OP9 stroma (day 2 of co-culture).
(C) hPSC (MIFF3) colony starting to differentiate on OP9 stroma (day 7 of co-culture).
(D) Differentiated hPSCs (MIFF3) at day 10 of OP9 stroma co-culture at time of harvest.
Microscope Olympus, CKX53. Scale bars were added using ImageJ software.
