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. 2021 Mar 31;2(2):100420. doi: 10.1016/j.xpro.2021.100420

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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MACS enrichment of CD34⁺ cells

Cells were harvested after OP9 culture on day 10 and enriched for hematopoietic progenitors using CD34 MACS beads. Cells in the CD34 positive and CD34 negative fraction were then stained for CD34 and analyzed by flow cytometry. Cells were gated for singlets, viability, and scatter profile. Histogram shows cells that express CD34 in the negative (gray) and positive (red) fraction respectively. After successful enrichment over 95% of the enriched cells are CD34⁺ and fewer than 5% CD34⁺ cells can be recovered from the CD34 depleted fraction.