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. 2021 Mar 31;2(2):100420. doi: 10.1016/j.xpro.2021.100420

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Flow cytometry analysis from approximately day 31 of co-culture

Cells were harvested after 3 weeks of MS5 co-culture and stained for surface markers.

(A) Cells were gated for singlets and scatter and then for viability (7AAD) and CD45 expression as indicated in the figure (top panel). Expression of CD19 and CD34 is shown within the 7AAD^-CD45⁺ fraction (second panel, left). The CD19 positive fraction is further gated for expression of CD10 and CD20 (right). Counting beads were used in this experiment and can be seen in the scatter plot in the top middle panel.

(B) Cells were gated for singlets and scatter (as in Figure 8A, plots not shown). Contour plot of viability (7AAD) and CD45 expression as shown in the figure (left). Further gating on 7AAD^-CD45⁺ cells for B lymphoid (CD19) and, within the CD19^- gate, myeloid (CD11b and CD14) markers are shown to the right.