Collagenase IV 10× stock |
Final concentration | Amount |
---|---|---|
Collagenase IV powder | 10mg/mL | 1g |
DMEM/F12 | 100ml |
Filter over a 0.22 μm membrane, aliquot and store at −20°C for up to 6 months.
To make 1× working solution dilute 10× in DMEM/F12. Always make fresh.