REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD10-BV421	BD	Cat# 562902; RRID: AB_2737879
CD11b-APC	Biolegend	Cat# 301310; RRID: AB_314162
CD14-PECy7	Biolegend	Cat# 325618; RRID: AB_830691
CD19-PE	Biolegend	Cat# 302254; RRID: AB_2564142
CD19-APC	Biolegend	Cat# 302212; RRID: AB_314242
CD20-BV510	BD	Cat# 563067; RRID: AB_2737985
CD31-APC	Biolegend	Cat# 303116; RRID: AB_1877151
CD33-BV786	BD	Cat# 740974; RRID: AB_2740599
CD34-PECy7	Biolegend	Cat# 343516; RRID: AB_1877251
CD43 FITC	BD	Cat# 555475; RRID: AB_395867
CD45-Alexa700	BD	Cat# 560566; RRID: AB_1645452
CD45RA-FITC	ThermoFisher	Cat# MHCD45RA01; RRID: AB_10373858
CD45RA-BV711	Biolegend	Cat# 304138; RRID: AB_2563815
CD127 (IL7R)-PE	Biolegend	Cat# 351304; RRID: AB_10720185
CD335-BV421	BD	Cat# 564065; RRID: AB_2738572
IgM-BV510	BD	Cat# 563113; RRID: AB_2738010
Human Fc Block	BD	Cat# 564220; RRID: AB_2869554
Chemicals, Peptides, and Recombinant Proteins
Human basic Fibroblast Growth Factor (hbFGF)	Amsbio	Cat# AMS-480-100; GenPept: P09038
Human Interleukin 3 (hIL-3)	PeProTech	Cat# 200-03; GenPept: P08700
Human FLT3 Ligand (hFLT3L)	PeProTech	Cat# 300-19; GenPept: P49771
Human Stem Cell Factor (hSCF)	PeProTech	Cat# 300-07; GenPept: P21583
Human Interleukin 7 (hIL7)	PeProTech	Cat# 200-07; GenPept: P13232
StemFit Basic04	Amsbio	Cat# SFB-504
Matrigel	Corning	Cat# 354277
Y27632 (Rock Inhibitor)	Cell guidance systems	Cat# SM02-10; CAS: 146986-50-7
Dimethyl Sulfoxide (DMSO)	Sigma-Aldrich	Cat# D2650; CAS: 67-68-5
Knock out replacement serum (KORS)	Gibco	Cat# 10828010
Gentle Cell Dissociation Reagent (GCDR)	Stem Cell Technologies	Cat# 07174
ReLeSR	Stem Cell Technologies	Cat# 05872
Qualified FBS	Gibco	Cat# 26140079
DMEM/F12	Gibco	Cat# 21331046
αMEM powder	Gibco	Cat# 12000014
Sodium Bicarbonate Solution (7.5%)	Sigma-Aldrich	Cat# S8761; CAS: 144-55-8
Distilled Water (tissue culture grade)	Gibco	Cat# 15230188
DPBS, Ca2+/Mg2+ free	Gibco	Cat# 14190169
Monothioglycerol (MTG)	Sigma-Aldrich	Cat# M6145; CAS: 96-27-5
Pencillin/Streptomycin	Gibco	Cat# 15140122
Collagenase, Type IV, powder	Gibco	Cat# 17104019
EDTA 0.5M	Sigma-Aldrich	Cat# E7889; CAS: 139-33-3
Trypsin 2.5%	Gibco	Cat# 15090046; CAS: 9002-07-7
Bovine gelatin solution (Type B, 2%)	Sigma-Aldrich	Cat# G1393; CAS: 9000-70-8
Gelatin from porcine skin (Type A)powder	Sigma-Aldrich	Cat# G1890; CAS: 9000-70-8
7-Aminoactinomycin D (7-AAD) (viability)	BD	Cat# 559925; RRID: AB_2869266
Trypan Blue (0.4%)	ThermoFisher	Cat# 15250061; CAS: 72-57-1
Critical Commercial Assays
QuadroMACS Separator	Miltenyi Biotec	Cat# 130-090-976
MACS LS Columns	Miltenyi Biotec	Cat# 130-042-401
CD34 MicroBead Kit, human	Miltenyi Biotec	Cat# 130-046-702; RRID: AB_2848167
Experimental Models: Cell Lines
ShiPS-MIFF1	University of Sheffield	https://hpscreg.eu/cell-line/UOSi001-A
ShiPS-MIFF3 (RRID:CVCL_1E70)	University of Sheffield	http://hpscreg.eu/cell-line/UOSi001-B
H1	WiCell	http://hpscreg.eu/cell-line/WAe001-A
OP9 stroma	ATCC	Cat# CRL-2749; RRID: CVCL_4398
MS5 stroma	DSMZ	Cat# ACC 441; RRID: CVCL_2128
Software and Algorithms
Flowjo		https://www.flowjo.com
Microscope	Olympus, CKX53	N/A
Scale bar software	ImageJ	https://imagej.nih.gov/ij/
Other
10-cm Tissue Culture Dish	Falcon	Cat# 353003
6-Well Cell Culture Plate	Corning	Cat# 3516
Cell Culture Flask 75cm2	Corning	Cat# 430641U
Disposable PES Filter Units (250 ml)	Fisherbrand	Cat# 15993297
Disposable PES Filter Units (500 ml)	Fisherbrand	Cat# 15913307