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| OP9-Maintenance media | Final concentration | Amount |
|---|---|---|
| αMEM from powder | 1× | 197.5ml |
| Qualified FBS | 20% | 50ml |
| Monothioglycerol (MTG) 1000× (100 mM) | 100μM | 250μl |
| Penicillin/Streptomycin | 1% | 2.5ml |
| Total | n/a | 250mL |
Filter over a 0.22 μm membrane and store at 4°C for up to 2 weeks.
