Fig. 3.

Depletion of ClsA in the absence of LpxM leads to a growth and morphological defect. (A) Growth curve following depletion of ClsA in the absence of lpxM. The strain clsA, lpxM/P_ara::clsA was grown under inducing conditions with 0.2% arabinose (+Ara, gray) or under repressing conditions with 0.2% glucose (−Ara, red). WT, single clsA, and single lpxM mutants were used as controls. The growth of a clsA, lpxM/P_ara::lpxM mutant was also observed in inducing conditions. Growth of indicated strains were monitored by OD₆₀₀ every 30 min. Error bars represent SEM from technical triplicate. (B) Serial dilutions of indicated strains were spotted on LB plates containing 0.2% glucose and grown at 37 °C. (C) Micrographs of cells at the 5 h time point of growth curve shown in A. (Scale bar, 5 μm).