Fig. 6.
Depletion of ClsA in the absence of LpxM decreases MsbA-mediated LPS transport. Cultures were grown to mid-log phase in either presence 0.2% arabinose (+) to induce plasmid expression or in 0.2% glucose (−) to repress plasmid expression. Cells were then labeled with 32Pi for one doubling, and the lipid A was isolated and separated by TLC and visualized by phosphorimaging. %LpxE-modified lipid A were determined by densitometry. Lipid A species are indicated, and the TLC is representative of three biological experiments.