Fig. 2.

Substitutions in its WDX domain destabilize TPLATE and affect TPC levels. (A) GFP-Trap of complemented TPLATE-GFP and WDXM2-GFP substitution lines. Input (I), flow through (FT), and bound (B) fractions were analyzed using an anti-GFP antibody. Protein degradation was quantified as the ratio between full-length protein and all smaller bands within each lane. (B) MS analysis following co-IP on complemented TPLATE-GFP and TPLATE-WDXM2-GFP lines. For each TPC subunit, the intensities of only those peptides that were present in all experiments (both baits and all in all replicas) were averaged and normalized to the values of the corresponding bait protein. SDs are based on three technical repeats.