Skip to main content
. 2021 Apr 5;118(15):e2023456118. doi: 10.1073/pnas.2023456118

Fig. 3.

Fig. 3.

Short-term heat treatment induces an incremental and reversible aggregation of TPLATE-WDXM2 in the cytoplasm. (A) Confocal images of root cells from TPLATE-GFP– and TPLATE-WDXM2-GFP–complemented lines subjected to normal conditions (20 °C, indicated as 35 °C 0 h) or heat treatment (35 °C 6 h). Arrowheads indicate the aggregated TPLATE-WDXM2 in the cytoplasm. (Scale bars, 20 µm.) (B) Confocal images of FDA and MitoTracker red (CM-H2XRos) staining in TPLATE- and TPLATE-WDXM2–complemented lines after 6 h heat treatment. (Scale bars, 25 µm.) (C–E) Confocal images (C and D) and quantification (E) of PM signal intensity in root cells from TPLATE-WDXM2-GFP–complemented lines before and after heat treatment. Seedlings after heat treatment were recovered in liquid 1/2 MS medium supplemented with or without 50 µM cycloheximide (CHX) at 20 °C. (Scale bars, 10 µm.) Numbers at the bottom represent the number of cells analyzed from at least six individual roots for each time point. Red circles represent the mean and letters represent significantly different groups (P < 0.001) evaluated by the Tukey multiple pairwise-comparisons test.