Fig. 3.

Adaptation of the SARS-CoV-2 replicon-based assay to high-throughput platform. (A) The plate view of GFP expression in replicon electroporated cells treated with compounds. The 293T cells were electroporated with T7 polymerase transcribed replicon RNA and incubated with control compounds in 384-well plates for 30 h. The plates were scanned using an Acumen eX3. (B) Correlation between the EC₅₀ values measured using freshly or frozen electroporated cells. A total of 27 control compounds was tested using either fresh or cryopreserved 293T cells electroporated by SARS-CoV-2 replicon RNA as described in Materials and Methods. The strength of correlation is determined by the Pearson test.