Figure 10.

miR-338-3p inhibited cell migration and invasion in TPC-1 and IHH-4 cells, which was reversed by SOX4 overexpression

(A) Cell survival was determined by a CCK-8 assay (reflected by OD₄₅₀ values) in TPC-1 and IHH-4 cells transfected with miRNA mimic, SOX4 overexpression plasmids, or both continuing for 3 days. (B) After transfection with a miRNA mimic, SOX4 overexpression plasmids, or both, a wound-healing assay was performed in TPC-1 and IHH-4 cells. (C) Cell migration was assessed by transwell migration assays, and miRNA mimic, SOX4 overexpression plasmids, or both were transfected into cells. Bar graphs were produced to illustrate the migratory cell numbers. (D) Cell invasion was assessed by transwell invasion assays. miRNA mimic, SOX4 overexpression plasmids, or both were transfected. (E) The protein levels of E-cadherin, vimentin, and fibronectin were detected by immunoblot analysis. n = 3, repetition = 3. Data indicate mean ± SD. Statistical analysis was conducted using a one-way ANOVA. All other groups were compared with the control group. ∗∗p < 0.01, versus control; ^##p < 0.01, compared with mimic group.