Figure 5.
The knockdown of circ_0001018 induced cell cycle arrest and promoted cell apoptosis in TPC-1 and IHH-4 cells
(A and B) Cell cycle profiles of TPC-1 and IHH-4 cell lines were analyzed by flow cytometry. circRNA-OE, sh-circRNA, miR-338-3p inhibitor, or sh-circRNA plus miR-338-3p inhibitor was transfected. Percentage of cells in the G1 phase is visualized as a bar graph. (C and D) Cell apoptosis was detected using flow cytometry. circRNA-OE, sh-circRNA, miR-338-3p inhibitor, or sh-circRNA plus miR-338-3p inhibitor was transfected. The upper left (UL) quadrant represents necrotic cells, lower left (LL) quadrant living cells, right upper (UR) quadrant late apoptotic and necrotic cells, and the lower right (LR) quadrant represents early apoptotic cells. Data indicate mean ± SD from at least three independent experiments and three repetitions. ∗p < 0.05, ∗∗p < 0.01, versus control; ##p < 0.01, versus sh-circRNA group.