Figure 6.
hsa_circ_0001018 promoted cell migration and invasion in TPC-1 and IHH-4 cells
(A) A wound-healing assay was performed in TPC-1 and IHH-4 cells, in which circ_0001018 overexpression, sh-circ_0001018, miR-338-3p inhibitor, or sh-circ_0001018 plus miR-338-3p inhibitor was transfected. Bar graphs were drawn using the derived data. (B) A transwell migration assay was performed in TPC-1 and IHH-4 cells, in which circ_0001018 overexpression, sh-circ_0001018, miR-338-3p inhibitor, or sh-circ_0001018 plus miR-338-3p inhibitor was transfected. Bar graphs were drawn using the derived data. (C) Cell invasion was assessed using a transwell invasion assay. Bar graphs were drawn to show the number of invading cells in every group. (D) The protein levels of E-cadherin, vimentin, and fibronectin were detected by immunoblot analysis. Data are in the format of mean ± SD. n = 3, repetition = 3. ∗p < 0.05, ∗∗p < 0.01, versus control; #p < 0.05, ##p < 0.01, versus sh-circRNA group.